Hello. I am involved in a project working towards identifying lipid residues in soil samples. One of the scientists advising me has suggested that I "spike" my control soil samples with a set of free fatty acids. These would be selected such that they represent fatty acids commonly found in this type of soil. I am tyring to reason this suggestion out. This is what I have come up with.
By spiking with the free fatty acids I would be able to easily identify the methyl ester derived after extraction, (I intend to extract and transesterify the freed fatty acids and then "spike" with the representative set). I think that the fatty acid will elute slightly before the derived methyl ester. I also think that "spiking" with known concentrations allows for easy quantitation of the fatty acids that were in the control.
So, do I just use the reference set to generate a standard to use for calibration when I run the sample set of unknowns?
Any feedback would be sincerely appreciated.