Hi all,
I got a problem with this:
Imagine a copy of moiety on the left side @ the black painted dot (right side).
Under unstable conditions (pH), the protein/peptide-Protectiongroup breaks away into: Protein + Protectiongroup (= C02 + Rest of Protectiongroup)
Let's assume that we're working under stable conditions --> how can i "cut off" the peptide +tag ? --> with tag i mean any moiety beside the PEG
-->so my aim is to have Peptide-NH-R whereas the R should always be the same
(it should be rerpoducible = if i take another batch with this protein+Protectiongroup and treat it with "the chemical solution" i wanna have the same result again..)
I hope you understand what i mean^^, im glad for any kind of support
regards
Diablooo
here's the link to the picture (enlarge on right bottom side)
http://www.tacticalxtreme.de/uploade/daten/1195829826_fmoc-pic.jpg