[baseball07]

Hi everyone.  I am covalently attaching an NH2 modified Oligo to a carboxylic acid terminated surface.  After the coupling, I was told to rinse the sample in PBS buffer with 1% SDS.  What is the role of SDS?  Is it to remove the unbound oligos?  What is the theory behind this?

Also, I just posted a topic in the organic chemistry forum but I think it is more relevant to post it here.  I am binding an NH2 modified oligo to a carboxylic acid modified surface using EDC and MES.  However I cannot find the recipe for MES buffer online.  Does anyone know what it is?  Is it ok to use just MES alone with EDC or does it have to be a buffer?  Whever I did a search online for MES buffer, it just came up with MES, calling it a buffering agent.  Can MES be used alone?