Im new to the forum so big hello !
Also I have not done any serious chemistry until this year when my thesis demands a small amount. Im basically interested in the transport of solutes across membranes and NH3 transport.
I started with a solution (Barth's buffer) consisting of :
23 mM NaCl
2.4 mM NaHCO3
1 mM KCl
0.33 mM Ca(NO3)2 * 4 H2O
0.41 mM CaCl2 * 2 H2O
0.82 mM MgSO4 * 7 H2O
5 mM Tris/HCl
This was adjusted from the starting ~70mOsm to 200mOsm with the addition of
NH4Cl (I dont have the exact mass added to acheive this but can go find it if needed!) and then adjusted to pH 6.5, 7.5 and 8.5.
So the question is : What is the concentration of NH3 (as opposed to NH4+)at each of the pHs ?