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Topic: What agent we use to colorate and de-colorate DNA when electrophoresis?  (Read 4148 times)

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Offline studytogether

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What agent we use to colorate and de-colorate DNA when electrophoresis?
« on: January 02, 2008, 01:33:09 AM »
we use EtBr to colorate DNA when electrophoresis

and what we use to de-colorate when electrophoresis?

thx 4 teaching
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Offline Alpha-Omega

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Re: What agent we use to colorate and de-colorate DNA when electrophoresis?
« Reply #1 on: January 02, 2008, 01:43:56 AM »
Way out of my area of expertise.  However, I do support DNAPac columns and it is my understanding Aldrich carries decolant agents for electrophoresis:

Stains and decolorants:  http://www.sigmaaldrich.com/catalog/search/TablePage/9623576


You may want to get this from someone who does DNA.....and uses electrophoresis
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Offline Arkcon

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Re: What agent we use to colorate and de-colorate DNA when electrophoresis?
« Reply #2 on: January 02, 2008, 08:17:57 AM »
Quote from: studytogether on January 02, 2008, 01:33:09 AM
we use EtBr to colorate DNA when electrophoresis

and what we use to de-colorate when electrophoresis?

thx 4 teaching

Ethidium bromide is a common stain for visualizing double stranded DNA in electrophoresis applications.  Destaining is accomplished by dilution, lots and lots of it.  What is your particular application?
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Hey, I'm not judging.  I just like to shoot straight.  I'm a man of science.
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