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Topic: Diluting a solution  (Read 3675 times)

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Offline wils0645

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Diluting a solution
« on: January 18, 2008, 11:57:32 AM »
I have a solution with 30% albumin (300 mg/ml), 0.85% NaCl (8.5 mg/ml) and 69.15% H2O.  I want to dilute with 0.9% NaCl to make a 100 mL solution.  My desired composition is 0.03% albumin, 0.85-0.9% NaCl, 99.07-99.12% H2O.  How much out of each solution should I take out and mix?

Offline AWK

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Re: Diluting a solution
« Reply #1 on: January 18, 2008, 12:07:04 PM »
Show your attempt.
AWK

Offline wils0645

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Re: Diluting a solution
« Reply #2 on: January 18, 2008, 12:17:24 PM »
If I have a 100 mL solution, 30 mg albumin is 0.03%.  850 mg NaCl is 0.85%.  100 mL H2O is 99.12%.  My problem is I'm trying to figure out with diluting with 0.9% saline instead of water.  That's what I'm confused about.  I don't believe I can use the eq. C1V1 = C2V2 can I?

(300 mg/mL) (?) = (0.3 mg/mL) (100 mL).  (?) = 0.1 mL or 100 uL.  So I would mix 99.9 mL NaCl, 0.1 mL albumin solution?  Thanks for the help.

Offline AWK

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Re: Diluting a solution
« Reply #3 on: January 18, 2008, 12:25:48 PM »
Good job
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Offline wils0645

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Re: Diluting a solution
« Reply #4 on: January 18, 2008, 12:29:40 PM »
That's right?  I surprise myself.

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