The flavonoid biochanin (http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?cid=5280373
) in fungus R.nigricans undergoes reversible
(bio)transformation to unknown product.
The fungus probably hydroxylates flavonoid (inserts additional OH group) with cytochrome P450 enzyme system or does another, unknown reaction. In any case, transformed biochanin after few hours converts back into biochanin (according to HPLC profiles). One posibility is reversible dimerisation or aryl shift or....The reaction proceeds in aquous medium with phosphate buffer pH 7, the concentration of flavonoid is 200 micromolar.
Has somebody any idea what REVERSIBLE proces is likely to happen on this compound?