On a cellulose TLC plate with the solvent being N-butanol/HOAc/water (4:1:1), I have to order the following amino acids from the one that will travel the highest on the plate to the one that will travel the least: Gly, Phe, Ala, Pro, Leu.
I ordered the amino acids: Leu, Pro, Ala, Phe, Gly, based on their hydropathy index values (more negative to more positive values). Is this correct?
I also have to explain why the amino acids would separate on the TLC plate in regards to the type of chromatography system (normal or reverse phase), and I would like some help explaining this. Thanks!