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Topic: anti-adhesive glass  (Read 6926 times)

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Offline anaxana

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anti-adhesive glass
« on: February 26, 2008, 07:32:05 AM »
 
dear all,

I have an in-vivo NMR setup, made out of a glass tube and glass capillaries and I use it for a bacterial cell suspension. After a while the cell suspension tends to form a biofilm on the walls of the tube or other parts of the setup and this is highly unwanted.

Do you think there is any treatment that I can do on my glass setup to diminish it's adhesive properties?

Another way would be to fluidize my cell suspension with a chemical compound that would form some kind of network that would repel cells from one another, but so far I haven't been successful with that, having tried already some dispersant agents.

If anyone could help me it would be great!

Thank you so much

Ana

Offline ARGOS++

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Re: anti-adhesive glass
« Reply #1 on: February 26, 2008, 07:58:42 AM »
Dear Anaxana;

I’m not very familiar with bacteria and cell suspension at all, but there is a technique to invert (hydrophobias) the surface of glass called “Silanization”.
To get an Idea about you may visit :    "Silanization” (with Links.)

For NMR it is possible it gives you only a sharp isolated additional Signal.
Maybe you can find also a corresponding “deuterated” Reagent.

I hope to have been of help to you.


Good Luck!
                    ARGOS++


Offline anaxana

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Re: anti-adhesive glass
« Reply #2 on: February 26, 2008, 09:09:38 AM »
 

   Dear Argos,

thank you so much for this idea. I am checking it out. I just have no idea if it's a simple process. Let's hope it works!  :)



Offline C6H8O7

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Re: anti-adhesive glass
« Reply #3 on: June 23, 2008, 05:23:23 AM »
This is the simple route.May want to do a bit of research to see if the teflon will contaminate anything

http://www.orioncoat.com/teflon-coated-glass-labware/

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