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Topic: retention mechanisms  (Read 3352 times)

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retention mechanisms
« on: January 29, 2008, 02:36:18 PM »
I have been reading alot of old journal of chromatography journals and I keep coming across the statement
"a linear decrease in log retention factor with increasing %organic means a partition mechanism is responsible for retention"-does anyone know why this

Offline LK

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Re: retention mechanisms
« Reply #1 on: March 16, 2008, 02:58:26 PM »
Hi Rosetta,

I guess they want to say that mechanism of interaction between the analyte and stationary phase is by partitioning... it is assumed to be partitioning when the plot of Log k' versus fraction of water (or % organic solvent) is linear

it is adsorption mechanism when Log k' versus Log fraction of water is linear...

by the way, did you find out about the pH of ammonium acetate in 50% MeOH? I'm very interested how it changes... did you measure? how did you do the calibration of pH meter?

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