I need to prepare antibiotic stock solutions of 10 mg/ml for Doxycycline hyclate and for Minocycline hydrochloride, crystalline (both purchased from Sigma). I would be really greateful if someone would help me with this.. I am a microbiologist, not a chemist or a pharmacist, so I have great troubles calculating this
. I have tried for a long time, but I always get too confused, so I really hope someone can help me (if so, only by suggesting some litterature)!
Minocycline hydrochloride, crystalline:
MW: 493.94, water by Karl Fischer: < OR = 10% (3MOL/MOL) , Purity by TLC: > OR = 98%, Elemental Analysis (anhydrous basis): 54.5 TO 57.3% Carbon 8.2 TO 8.8% Nitrogen, Potency (anhydrous basis): 890 TO 950 micrograms minocycline/mg
MW: 512.94, water by Karl Fischer: NMT 5%, Solvent content: NMT 7% Ethanol by Gas Chromatography, Elemental analysis(anhydrous basis): 5.5 TO 6.1% Nitrogen, Purity by TLC: 98% minimum