April 13, 2024, 08:38:01 AM
Forum Rules: Read This Before Posting


Topic: HPLC - Difference between binary and quaternary pumps?  (Read 28984 times)

0 Members and 1 Guest are viewing this topic.

Offline mobfesk2

  • Regular Member
  • ***
  • Posts: 19
  • Mole Snacks: +0/-0
HPLC - Difference between binary and quaternary pumps?
« on: March 20, 2008, 06:58:23 PM »
Hi Chem forumers,

This might sound like a dumb question but does anyone know the difference between binary and quaternary HPLC?

Thanks!
Sugar chemistry... sweet or sour?

Offline Arkcon

  • Retired Staff
  • Sr. Member
  • *
  • Posts: 7367
  • Mole Snacks: +533/-147
Re: HPLC - Difference between binary and quaternary pumps?
« Reply #1 on: March 20, 2008, 10:08:10 PM »
Hi Chem forumers,

This might sound like a dumb question but does anyone know the difference between binary and quaternary HPLC?

Thanks!

Yes, that generally refers to the number of solvent inlet lines, a binary has two, and can program methods that mix two eluents, either isocratic or gradient, look those terms up.  A quaternary can mix 4 eluents.  You might use the extra eluents for a very complicated mixture, or a 3 component gradient, or to include wash solvents, or to just have 2 pairs of eluents, so you can pick and choose between 2 or more separations, without changing eluent bottles.

The HPLC itself can have one pump, which mixes from it's eluent bottles before the pump.  Or it can have one pump per eluent, but that gets pricey, especially at 4 eluents.
Hey, I'm not judging.  I just like to shoot straight.  I'm a man of science.

Sponsored Links