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Topic: Membrane Potential  (Read 4952 times)

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Offline DUDE778

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Membrane Potential
« on: March 23, 2008, 09:16:27 PM »
A biological cell is immersed in a 70. mM solution of NaCl at 37°C. The cell's membrane is permeable only to Na+ ions. When the system reaches equilibrium, a potential difference of 72.2 mV is measured between the inside and the outside of the cell, where the inside has the higher (more positive) potential. Calculate [Na+] inside the cell, assuming the amount of Na+ ions transferred is negligible compared to the total amount of Na+ ions in the solution.

Can someone give me a hand with this

Offline Padfoot

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Re: Membrane Potential
« Reply #1 on: March 23, 2008, 09:53:07 PM »
a potential difference of 72.2 mV is measured
If you know the equilibruim potential, what piece of info does the Nernst eqn give you?

assuming the amount of Na+ ions transferred is negligible compared to the total amount of Na+ ions in the solution.
What can you say about the extracellular concentration?  Has it changed?

Also, don't forget your units are mM!

EDIT: spelling  ;)



« Last Edit: April 25, 2008, 09:01:45 PM by Brett »

Offline DUDE778

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Re: Membrane Potential
« Reply #2 on: March 23, 2008, 10:11:10 PM »
did i do it right..
72.2mV=61log[Na out] / [Na in]
i got 4.586M

Offline Padfoot

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Re: Membrane Potential
« Reply #3 on: March 23, 2008, 10:31:05 PM »
did i do it right..
Yep
Though units are mM.

Also, don't forget your units are mM!
 Lordy Lord...   ::)

72.2mV=61log[Na out] / [Na in]
Note that the Nernst eqn is: Eion= (-RT/zF)ln([ION]i/[ION]o)
which corresponds to -61log([ION]i/[ION]o) in this case.
(though mathematically your eqn is still right since log(a/b)=-log(b/a))

Edit: http://www.chemicalforums.com/index.php?topic=20830.msg78975#msg78975

That was 4 months ago  ::)
Sheesh  :P
« Last Edit: April 25, 2008, 08:59:42 PM by Brett »

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