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Topic: e.coli test  (Read 7998 times)

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Offline dionl

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e.coli test
« on: April 30, 2008, 10:29:07 AM »
Hi, are there anybody who knows how can I prepare a test to carry out the total e.coli form?
I know sodium thiosulfate solution is used , but I don't know the amount of sodium thiosulfate :-\

Offline eugenedakin

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Re: e.coli test
« Reply #1 on: April 30, 2008, 07:54:24 PM »
Hello dionl,

Sorry, I am having a difficult time understanding your question:

Quote
are there anybody who knows how can I prepare a test to carry out the total e.coli form?

What are you trying to accomplish: grow cells? Identify type? Determine amount? Is this in wastewaster?

Thanks for your help.

Sincerely,

Eugene
There are 10 kinds of people in this world: Those who understand binary, and those that do not.

Offline Gerard

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Re: e.coli test
« Reply #2 on: April 30, 2008, 09:46:11 PM »
tip read: standard test methods for water and wasterwater methods....
but there are different methods for doing that...
culturing needs agar....
if you want to determine your water is contaminated you can use the plate counting methods or the color reaction method
or you can buy a water testing kit that is sold at you local water testing agency
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Offline dionl

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Re: e.coli test
« Reply #3 on: May 06, 2008, 03:03:28 AM »
Hello dionl,

Sorry, I am having a difficult time understanding your question:

Quote
are there anybody who knows how can I prepare a test to carry out the total e.coli form?

What are you trying to accomplish: grow cells? Identify type? Determine amount? Is this in wastewaster?

Thanks for your help.

Sincerely,


Eugene
Hi Eugene, I am trying to prepare a coli test to carry out coli form from the water? so I am trying a test for water ;) but I am not sure about the amounts of sodium thiosulfate in solution ? ???not growing :-\

Offline eugenedakin

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Re: e.coli test
« Reply #4 on: May 06, 2008, 08:09:26 PM »
Hi dionl,

Artificial student is quite right.  The Standard Methods for water and wastewater have these approved tests.  Be careful when performing these tests on your own, as there are legal implications to be considered.

I hope this points you in the right direction.

Sincerely,

Eugene
There are 10 kinds of people in this world: Those who understand binary, and those that do not.

Offline dionl

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Re: e.coli test
« Reply #5 on: May 07, 2008, 04:33:33 AM »
Hi dionl,

Artificial student is quite right.  The Standard Methods for water and wastewater have these approved tests.  Be careful when performing these tests on your own, as there are legal implications to be considered.

I hope this points you in the right direction.

Sincerely,

Eugene

thank you I understand.. instead of preparing I will find it  ::)

Offline Gerard

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Re: e.coli test
« Reply #6 on: May 11, 2008, 02:05:41 AM »
ill give you a non-approved way of testing if your water is contaminated...
first put a small amount of table sugar in your water sample..
incubate for a day at 35 degress celsius and aerate the water,afterwards check for turbidity, if your water is turbid try dropping a 500 mg of powdered penicillin into your water if the turbidity faeds then it is bacteria infested..
"Charles! Charles! That's it Mr. Charles Darwin get out of this room, I told you once and I told you twice not to tease your fellow Mr. Arrhenius!"

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