I'm working on my final lab report for the year and we are asked to plot enzyme kinetics using 4 different plot types.

Michaelis-menten

Lineweaver-burk

Eadie-Hofstee

Hanes-wolf

For these plots we need to determine which is the one that gives the best data. Now I feel that michaelis-menten is the best and am working on the explanation. I feel that the lineweaver-burk has flaws in that you need to extrapolate the data far back to the y-axis giving the 1/Vmax some variance depending on the fit of the line. Also the fact that the largest values are actually the lowest readings, this puts a lot of dependence on the accuracy of the spectrophotometer used. As for the other two, most of the people i talk to can't give me any insight about those. Most haven't heard of them (like me never heard of it till this lab). So I someone could shed some light on these types of plots. Pros/Cons?, thanks.

Also we need to evaluate the Kcat from our Vmax and Km...I can find info about how to solve it but I need to have "prism 5", which i don't have or even know what it is. So if someone can give me a simple equation or something I can derive it from would be very appreciated.

I just want to be done with this. I do love a teacher that wants you to report on an item that you have never learned about. Thanks in advance for you help

YU