August 08, 2020, 05:34:08 PM
Forum Rules: Read This Before Posting


Topic: 5 Kb and 4.95 kb dsDNA strand exchange???  (Read 4478 times)

0 Members and 1 Guest are viewing this topic.

Offline JoshA

  • Very New Member
  • *
  • Posts: 1
  • Mole Snacks: +0/-0
5 Kb and 4.95 kb dsDNA strand exchange???
« on: June 03, 2008, 06:57:00 PM »
Hi everyone,
 I have been looking in the literature but I have not been able to find anything on this subject. I have PCR'ed a 5 kb DNA and a 4.95 kb DNA. The 5 kb and 4.95 kb are identical except that the 5 kb is just 50 bp's longer.
  If I put both of these DNA's (5 kb and 4.95 kb) in a test tube and heat them to 100 C for and 1-2 hours, do you think I could get some of the strands from the 4.95 and 5 to swap? This would give me some dsDNA with a 50 bp overhang, which is what I want.
  If I can not do this, is there any other way I can go about it? Thanks-Josh

Sponsored Links