[yitriana]

The absorption of UV light at 280 nm by protein is mainly due to trptophan and tyrosine. If you are studying a protein with molecular weight of 26 kDa and you need to make a solution that is 1 mg/mL of the protein. Unfortunately, you don't have lots of protein to make a lot of solution, and it is difficult to weight of 1 mg! What could you do if you had a 1 cm path length cuvette and a UV-Vis spectrophotometer?

molar decadic absorption coefficients for tryptophan and tyrosine are 5690 and 1280 L/cm-mol.

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why do you assume that there are 6 tyrosines and 2 tryptophan  molecules in the protein?

[ARGOS++]

Dear yitriana;

Please show us your work, that we can guide you!   ─    And would you please read:  "Forum Rules" (4.)

Good Luck!
                    ARGOS⁺⁺

[typhoon2028]

I don't understand why you need a solution with a concentration of 1 mg/mL.

Concentration should be proportional to absorbtion.

[Arkcon]

yitriana:, can you start figureing this one out, by writing Beer's law out as a formula, and seeing what pieces of info you have, and what are just implied, and what pieces, you really don't even need, especially given typhoon2028:'s hint?