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### Topic: How do I prepare this solution? Dilution? CV=CV?  (Read 3358 times)

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#### 12179

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##### How do I prepare this solution? Dilution? CV=CV?
« on: April 06, 2009, 10:58:19 PM »
I need to prepare 50 mL of "aqueous solution 50mM in SDS and 30 mM in NaCl"

MW of SDS = 288.38g/mol
MW of NaCl=58.44 g/mol

How do I prepare this? I think aqueous means I should dissolve it in water. So SDS in water, then NaCl in water...and then combine it to be 50mL? But then how much water do I dissolve in each? How many grams of each?

Do I prepare 25mL of 50mM SDS and then 25mL of 30mM NaCl and combine them?
Does it matter if it's 10mL of 50mM SDS and 40 mL of 30mM NaCl?

#### sjb

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##### Re: How do I prepare this solution? Dilution? CV=CV?
« Reply #1 on: April 07, 2009, 03:38:46 AM »
If you had 40 ml of a 50 mM solution of SDS, then diluted it to 50 ml, is the solution of SDS still 50 mM?

#### 12179

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##### Re: How do I prepare this solution? Dilution? CV=CV?
« Reply #2 on: April 07, 2009, 07:28:48 PM »
Thanks. Figured it out.