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Topic: How do I prepare this solution? Dilution? CV=CV?  (Read 3357 times)

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Offline 12179

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How do I prepare this solution? Dilution? CV=CV?
« on: April 06, 2009, 10:58:19 PM »
I need to prepare 50 mL of "aqueous solution 50mM in SDS and 30 mM in NaCl"

MW of SDS = 288.38g/mol
MW of NaCl=58.44 g/mol

How do I prepare this? I think aqueous means I should dissolve it in water. So SDS in water, then NaCl in water...and then combine it to be 50mL? But then how much water do I dissolve in each? How many grams of each?


Do I prepare 25mL of 50mM SDS and then 25mL of 30mM NaCl and combine them?
Does it matter if it's 10mL of 50mM SDS and 40 mL of 30mM NaCl?

Offline sjb

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Re: How do I prepare this solution? Dilution? CV=CV?
« Reply #1 on: April 07, 2009, 03:38:46 AM »
If you had 40 ml of a 50 mM solution of SDS, then diluted it to 50 ml, is the solution of SDS still 50 mM?

Offline 12179

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Re: How do I prepare this solution? Dilution? CV=CV?
« Reply #2 on: April 07, 2009, 07:28:48 PM »
Thanks. Figured it out.

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