I am having trouble with the Megazyme kit (K-SUFRG) for sucrose, glucose, fructose. The kit is specific for gluctose and fructose, but sucrose is not measured independently. The response for glucose (ΔAgluc) is subtracted from the response for sucrose (ΔA total glucose) and this is resulting in low and even negative values for sucrose. I am trying to measure gluc, fruc, suc in sweet potato extracts and sucrose is expected to be the predominant sugar. But my values for sucrose are usually lower than fructose and glucose.
Is anyone familiar with this kit? Please help.