July 22, 2024, 04:30:11 PM
Forum Rules: Read This Before Posting

Topic: UV Spec Iron calibration curves  (Read 3357 times)

0 Members and 1 Guest are viewing this topic.

Offline tchristides

  • New Member
  • **
  • Posts: 3
  • Mole Snacks: +0/-0
UV Spec Iron calibration curves
« on: June 13, 2009, 05:55:08 PM »
I have been trying to make calibration curves for a single beam UV Spec using ferric chloride and ferrous sulphate.
My  calibration concentration range is 10-100,000 micromolar, and I have adjusted the pH of my solutions to 7 using NaOH (or tried to anyway).
The problem I am having is that at concentrations of ferrous sulphate greater than 100 micromolar I am getting percipitation of the iron as pH increases - and I don't even think its ferrous iron anymore as it changes color to orange/red - ferric iron?
If the expected levels of Fe++&Fe+++ in my unknowns are 10-100 micromolar, can my calibration curves also be in that range?
Also, I'm assuming that absorbtion will change for a given valence of iron as pH changes?
Any insights would be very much appreciated.

Sponsored Links