Hi, my question deals with dilutions and the m1v1=m2v2 equation. I want to dilute a 1000ppb (1ppm) solution down to 100, 150, 200, 250, and 300 ppb for a calibration curve. I have five test tubes for each sample all of which I fill with 10 mL of nanopure DI water. If I want a 1, 2, 3, 4, 5 ppb calibration I would add 10uL, 20uL, 30uL, and so on. However, my problem with the dilutions to 100ppb-300ppb is if m1v1=m2v2 still holds true due to the large dilution. Specifically, for example making the 300pbb, would I use (1000ppb)v1=(300ppb)(10mL) or would I use (1000ppb)v1=(300ppb)(13mL) since 13 would be the final volume. thanks
James