August 05, 2020, 03:43:50 AM
Forum Rules: Read This Before Posting


Topic: Buffers question  (Read 1688 times)

0 Members and 1 Guest are viewing this topic.

Offline klasnikov

  • New Member
  • **
  • Posts: 8
  • Mole Snacks: +0/-0
Buffers question
« on: October 01, 2009, 07:03:07 PM »
A question from my text book reads:

"To undergo a reaction it is necessary to have 0.25ml of an enzyme at a concentration of 0.2 mgml-1 in the presence of 0.3 mM enzyme and 200 mM NaCl all in the same buffer. The stock solutions of enzyme, substrate and NaCl are 4mgml-1, 40mM and 1M all in the same buffer. What volumes of buffer and stock are required for each assay?"

Any ideas how to tackle this?

I posted this in the high school section but I feel it's more suited to here.

Offline Borek

  • Mr. pH
  • Administrator
  • Deity Member
  • *
  • Posts: 25874
  • Mole Snacks: +1692/-401
  • Gender: Male
  • I am known to be occasionally wrong.
    • Chembuddy
Re: Buffers question
« Reply #1 on: October 01, 2009, 07:17:29 PM »
Nah, it will get enough attention in HS.
ChemBuddy chemical calculators - stoichiometry, pH, concentration, buffer preparation, titrations.info, pH-meter.info

Sponsored Links