The wording in your text is unclear :
the way it reads is that 0.58 is your calculated molar absorption coefficient of hemoglobin at the wavelength of maximum absorption, however from your calculations it is the Absorbance measurement made during the experiment?
"We were also told the absorption of a solution of hemoglobin which was 0.11g/L" Have you told us what it was? Is this the 0.58 value?
If you were working at ~414 nm you're not that far away with your calculated result.
You're wrong about your concentration assumption. If I give you a solution of xg/mL and tell you it is made by diluting a solution of y g/mL by a factor of 100, what is the relationship between x and y?
In your example you've been given a diluted sample and a dilution factor and asked to calculate the concentration before dilution.
Hey, thank you for your response. Hope i can clarify what i said in my first post.Method of experiment:
The absorption of a solution of hemoglobin (0.11g/L) in distilled water was recorded at 20 nm intervals from 340 nm to 540 nm using a spectrophotometer. After plotting my values on a graph of absorption (Y-axis) against wavelength ( x-axis), i found out (from my graph) that the maximum absorption was at 0.58 at a wavelength of 410nm.1) The question:
Given the molecular weight of hemoglobin being 65000, calculate the molar absorption coefficient of hemoglobin at the wavelength of maximum absorption.2)
For my second part of the question about the dilution, is it right for me to conclude that since the concentration i calculated was with dilution i need to multiply the answer by 1x10^3 ( because the dilution was 1 in 1000)?
Hope i clarified it a bit better.
Thank you again,