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Topic: Protein Unfolding: "microview" entropy  (Read 2362 times)

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Offline fender1289

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Protein Unfolding: "microview" entropy
« on: November 19, 2009, 11:45:30 PM »
This is the equilibrium for the unfolding/folding of a protein at high temperatures.
Folded<-->Unfolded
For a given sample,
At 57°C, 50% of the sample is folded.
at 64°C, 90% of the sample is unfolded.

(A)   Calculate ΔH° of unfolding. (Assume ΔH and ΔS remain constant through the temperature range under consideration.)
(C)   Calculate ΔS° of unfolding

Not sure where to start here. I think this will have something to do with the number of available configurations for the protein, i.e. 2^n  where n is the number of protein molecules.

∆S=Kb ln(Ω1/Ω2)? I just need some help on where to start with this one. Thanks.

Offline Yggdrasil

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Re: Protein Unfolding: "microview" entropy
« Reply #1 on: November 20, 2009, 12:21:50 PM »
A good starting point is to calculate the equilibrium constant for folding at the two temperatures, then relate that to the change in free energy of folding (ΔG).

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