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Topic: proper staining technique.  (Read 4797 times)

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Offline 8888kirbz

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proper staining technique.
« on: November 23, 2009, 07:07:28 PM »
What is the proper procedure in staining blood smears with acridine orange?

Offline JGK

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Re: proper staining technique.
« Reply #1 on: November 24, 2009, 12:10:04 PM »
Check the bottom of Page one [url][http://www.pmlmicro.com/assets/TDS/885.pdf/url]
Experience is something you don't get until just after you need it.

Offline 8888kirbz

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Re: proper staining technique.
« Reply #2 on: November 27, 2009, 08:18:31 AM »
that's exactly what i've done sir... but some of my slides having blood smears has its cells not so clearly stained. I dont know what went wrong. Will it be ok if i'll jus restain those slides?

Offline JGK

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Re: proper staining technique.
« Reply #3 on: November 27, 2009, 03:40:51 PM »
To be honest I wouldn't know the last time I satined blood smears was in the 1980s (using H&E stain).

However, I wouldn't recommend restaining all of the slides at once. Try one or two first to see if they will take more stain.
Experience is something you don't get until just after you need it.

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