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Topic: Why?What?How?  (Read 3925 times)

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asqa

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Why?What?How?
« on: July 06, 2005, 02:31:34 AM »
If we take a 1:1 weight ratio of two solvent and it is analysed by GC then we will not get 50:50? -Reason

Same things holds good in HPLC also.Why?

Please Substantiate?

Offline DrCMS

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Re:Why?What?How?
« Reply #1 on: July 06, 2005, 03:56:05 AM »
If you analyse them and just look at area% then you won't get 50:50 due to the varying reponse of the two solvents in FID or UV detection.

asqa

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Re:Why?What?How?
« Reply #2 on: July 06, 2005, 04:23:32 AM »
Dr. CMS If we take total max plot instead of taking absorbance at wavelength maxima in uv whether we may get 50:50 ?

Offline DrCMS

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Re:Why?What?How?
« Reply #3 on: July 06, 2005, 10:46:23 AM »
That may be better but your two solvents will not have identical total absorbances.  You'd need to calibrate with standards to get the correct answer.

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