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Topic: Nitrite Test  (Read 2539 times)

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Offline weigewg1994

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Nitrite Test
« on: December 21, 2009, 02:59:13 PM »
If I wanted to detect the different amounts of nitrites in samples of spinach after storage, what should i do?

I thought of soaking the spinach in water then conduct a basic nitrite test. The nitrite test would be like this:

1. Add 1M sulfuric acid to sample
2. Add iron (II) sulphate to sample
3. A brown solution should form since

2NaNO2 + 2 FeSO4 + 3 H2SO4 --> Fe2(SO4)3 + 2 NaHSO4 + 2 H2O + 2 NO

Is this correct? Since nitrites are supposed to oxidize the iron (II) into iron (III), hence the change of colour. Will there be problems with my experiment? What other nitrite tests are available?

Any advice would be much appreciated!

Offline Golden_4_Life

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Re: Nitrite Test
« Reply #1 on: December 22, 2009, 02:15:26 PM »
Be sure to include at least three conc'n levels of pure nitrite STDs {standards} in your assay; also, you may want to use a colorimeter to quantify and differentiate the slight variance in sol'n color change as a result of the oxidation.
Golden4Life

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