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Topic: Pka determination from graph  (Read 4586 times)

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Offline spas105

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Pka determination from graph
« on: February 28, 2010, 07:43:27 PM »
Ok so i have a graph of pH vs log([HPO4^2-]/[H2PO4-])
Then it says to perform a least squares fit. I did that and then it asks to determine the pka from this graph. I dont understand how i do that from the graph.  I know how to use the equation but do not understand how to use the graph.

Offline Borek

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Re: Pka determination from graph
« Reply #1 on: March 01, 2010, 02:16:01 AM »
Linear regresion?

Take a look at the Henderson-Hasselbalch equation - it is exactly what you need.
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