Of course I would guess no, but I'm struggling to explain why- we need to "include techniques that either were already performed or should be done to support your claim." We did this in the lab:
Salt fractionation with 35% ammonium sulfate
Salt fractionation with 57% ammonium sulfate
Methanol fractionation and heat treatment
I don't see how any of these can give hints as to the final purity of the enzyme, and I can't seem to find much in outside sources. If someone could even give me a hint (like what terms I should use in a search engine or a book index), it would help A LOT
My only guess is that, in the context of our lab, we still haven't done absolutely everything we can to purify the enzyme. In sources I saw a lot of people mentioning ion separation and gel electrophoresis as other ways to purify an enzyme. Could I say that our enzyme is not 100% pure because these techniques haven't been performed? Or is there something I'm just missing?