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Topic: chromatographic integration methods  (Read 4471 times)

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Offline Ibin-Huan

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chromatographic integration methods
« on: May 09, 2010, 03:00:14 PM »
Hi guys!!!!!!!!!!!!!!

Does anyone can provide me PDF file, power poin or any form else that explain how to estimate manually the concentration of an analyte in GS by both using peak area and peak hight. I know that it can be done automticaly by GC instrument but I want to understand it.
Thank you
« Last Edit: May 09, 2010, 05:34:50 PM by Borek »

Offline Borek

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Re: chromatographic integration methods
« Reply #1 on: May 09, 2010, 05:39:55 PM »
I think any book on instrumental analysis should explain it.
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Offline Golden_4_Life

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Re: chromatographic integration methods
« Reply #2 on: May 10, 2010, 10:37:02 AM »
1. get a pair of scissors and carefully,precisely cut out the peaks of your standard and test samples.
2. place the paper cut outs on a 4-place digital balance.
3. carefully measure the weights of the Peaks to 0.0001 gram for both the reference Standard and the test samples.
4. compute the concentration of your test samples by the usual ratio method in comparison to your Standards.
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Offline crosemeyer

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Re: chromatographic integration methods
« Reply #3 on: May 10, 2010, 01:20:58 PM »
1. get a pair of scissors and carefully,precisely cut out the peaks of your standard and test samples.
2. place the paper cut outs on a 4-place digital balance.
3. carefully measure the weights of the Peaks to 0.0001 gram for both the reference Standard and the test samples.
4. compute the concentration of your test samples by the usual ratio method in comparison to your Standards.

Awesomest thing I've read all day!   :D

Offline JGK

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Re: chromatographic integration methods
« Reply #4 on: May 10, 2010, 01:58:23 PM »
1. get a pair of scissors and carefully,precisely cut out the peaks of your standard and test samples.
2. place the paper cut outs on a 4-place digital balance.
3. carefully measure the weights of the Peaks to 0.0001 gram for both the reference Standard and the test samples.
4. compute the concentration of your test samples by the usual ratio method in comparison to your Standards.

I remember doing that when we had to validate our computing integrators against the old chart recorders back in the day
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Offline Golden_4_Life

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Re: chromatographic integration methods
« Reply #5 on: May 10, 2010, 02:04:27 PM »
Heheh--yes this is an antiquated method of quantification.
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