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Topic: enol and keto two peaks in HPLC measurement?  (Read 3768 times)

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Offline Ray_yu

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enol and keto two peaks in HPLC measurement?
« on: May 26, 2010, 07:02:45 AM »
do enol and keto forms of a same compound show two peaks in the HPLC measurement??? or only one???

In several paper they described they do have two at very low temperature (-60C)
some of them did the detection by UV spectrophotometer instead of HPLC

Could anyone give some hints>??
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Offline JGK

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Re: enol and keto two peaks in HPLC measurement?
« Reply #1 on: May 27, 2010, 03:07:09 PM »
I used to perform a method where the analyte exhibited Keto-Enol Tautomerism, there was one analyte peak but the peak response was highly variable until the tautomerism reached equilibrium
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