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Topic: DNA gel electrophoresis  (Read 5451 times)

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lufthoved

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DNA gel electrophoresis
« on: August 11, 2005, 06:35:09 AM »
Hi  everyone...

Im working with DNA and PCR and wanted to test my amplicons. Therefore I made a gel.

Actually it turned out very good but I was wondering how to improve my result.

1. There a large white shadow behinds my band (Im using EtBr and dye). How can I reduce this (or better yet remove it)?

2. Should I run longer time, so the bands will end up post the shadow?

Pardon my english (I come from Denmark)...

Best wishes
lufthoved

Offline sdekivit

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Re:DNA gel electrophoresis
« Reply #1 on: August 11, 2005, 11:56:47 AM »
i think you need to run longer

lufthoved

  • Guest
Re:DNA gel electrophoresis
« Reply #2 on: August 12, 2005, 05:08:32 AM »
You think the band will be better then, and the shadow smaller?

Kira

  • Guest
Re:DNA gel electrophoresis
« Reply #3 on: August 24, 2005, 06:51:35 AM »
1.You can try to reduce the amount of ethBr.
2.Sometimes is it enough to clean the photo-table.
3.Running the gel longer makes no sense in my opinion.

best wishes kira

lufthoved

  • Guest
Re:DNA gel electrophoresis
« Reply #4 on: August 29, 2005, 03:24:13 AM »
Hi, all. Thank you for your answers.


I was wondering if it would be better could mix EthBr with the samples and apply to the wells instead of mixing in the gel?


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