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DNA gel electrophoresis

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lufthoved:
Hi  everyone...

Im working with DNA and PCR and wanted to test my amplicons. Therefore I made a gel.

Actually it turned out very good but I was wondering how to improve my result.

1. There a large white shadow behinds my band (Im using EtBr and dye). How can I reduce this (or better yet remove it)?

2. Should I run longer time, so the bands will end up post the shadow?

Pardon my english (I come from Denmark)...

Best wishes
lufthoved

sdekivit:
i think you need to run longer

lufthoved:
You think the band will be better then, and the shadow smaller?

Kira:
1.You can try to reduce the amount of ethBr.
2.Sometimes is it enough to clean the photo-table.
3.Running the gel longer makes no sense in my opinion.

best wishes kira

lufthoved:
Hi, all. Thank you for your answers.


I was wondering if it would be better could mix EthBr with the samples and apply to the wells instead of mixing in the gel?

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