Im working with DNA and PCR and wanted to test my amplicons. Therefore I made a gel.
Actually it turned out very good but I was wondering how to improve my result.
1. There a large white shadow behinds my band (Im using EtBr and dye). How can I reduce this (or better yet remove it)?
2. Should I run longer time, so the bands will end up post the shadow?
Pardon my english (I come from Denmark)...