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Topic: Why should IPTG induction be carried out in the dark?  (Read 5095 times)

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Offline kristinase

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Why should IPTG induction be carried out in the dark?
« on: August 13, 2010, 12:16:54 PM »
In several protocols for protein expression in recombinant E coli with IPTG induction they put the cultures in the dark after induction and I was just wondering why this is done. Thanks!
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Offline Wreath

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Re: Why should IPTG induction be carried out in the dark?
« Reply #1 on: August 15, 2010, 05:09:01 PM »
because IPTG is degraded by light.
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