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Topic: Assay for enzymic activity the organic way  (Read 3282 times)

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Assay for enzymic activity the organic way
« on: August 16, 2005, 11:15:56 AM »
pH adjusting is necessary because it will create a favorable
environment which such substance, for instance enzyme, will work
properly.
For example, Tris-HCl should be adjusted into pH 7.5 to get the
optimal DNase and RNase activity in your assays.

What happens if we're to alter the pH. Does it mean that inductiion
and resonance does not operate efficiently rendering the protein in
question to cross fire all the electrophiles and nucleophiles in the
wrong order and sequences such tat we can get a more than active
enzyme?

Very basic or acidic medium of pH?

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