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Topic: flame aas method for glukoz and non-glukoz dialysşs solution  (Read 4246 times)

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Offline vistapro

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flame aas method for glukoz and non-glukoz dialysşs solution
« on: September 03, 2010, 04:23:11 PM »
 i need flame aas method for glukoz and non-glukoz dialysşs solution especialy for dialysis solution with glukoz

Offline crt

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Re: flame aas method for glukoz and non-glukoz dialysşs solution
« Reply #1 on: September 07, 2010, 02:58:16 AM »
You cannot measure glucose in a direct way with AAS. You need an atom which you can excitate in the flame. Carbon and oxygen do not belong to these atoms. Besides this you use usually acetylene, air and/or nitrous oxide to feed the flame...

Offline Stepan

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Re: flame aas method for glukoz and non-glukoz dialysşs solution
« Reply #2 on: September 07, 2010, 04:20:48 PM »
Agree with CRT unless you want to test for NaCl

Offline crt

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Re: flame aas method for glukoz and non-glukoz dialysşs solution
« Reply #3 on: September 07, 2010, 05:59:12 PM »
If you want to measure Na, then use millipore water and KNO3 to have a stable signal and to suppress Na+.

Offline nilakkal

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Re: flame aas method for glukoz and non-glukoz dialysşs solution
« Reply #4 on: September 15, 2010, 12:51:38 AM »
You are not specific in your question. In these solutions which metals are you interested in? If you are interested in sodium, the answer is as follows. First of all glucose is highly soluble in water. So you can prepare your solutions in deionized water itself. keep in mind in the presence of high concentrations of mineral acids, the sodium signal is reduced. Now you can use air-acetylene flame and to control the ionization interferences add 0.1% or more of pottassium as chloride.

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