May 25, 2020, 02:22:01 AM
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Topic: What accounts for the discrepancy in peak heights on UV-Vis spectroscopy?  (Read 1805 times)

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Offline DutchGirl13

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I am looking at graphs made by a UV-Vis spectrophotometer of a HoCl3 solution. At a slit width of 0.04 nm, the peaks are sharp and high. When the same solution was analyzed with a slit width of 5.00 nm, the peaks were wider and shorter. In one case, the 5-nm peak was about five-eighths the size of the 0.04-nm peak. I am sure it has something to do the slit opening size, but i am not sure how to connect it. I think it goes something like this: the broader slit width allows in more light, so it seems the light comes from a larger range of wavelengths and is not concentrated as much at one wavelength. Is this close to reality?

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