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Topic: can polysaccharide be seen by uv detector  (Read 3894 times)

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Offline HB

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can polysaccharide be seen by uv detector
« on: January 19, 2011, 03:38:04 PM »
I have extracted a polysaccharide from an algae, can it be detected with UV detector or react with any chemical compound so that it can be detected with UV detector or it is impossible

Offline JGK

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Re: can polysaccharide be seen by uv detector
« Reply #1 on: January 20, 2011, 01:47:11 PM »
Sugars have very poor chromophores and are not detected well by UV.

Most chromatography of sugars uses either Refracive Index (RI) or evaporative light scattering (ELS) detection.
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