[Polleke]

Hallo all,

I have no idea of anyone here knows something about medium for the in vitro culture of plants?
Anyway: I had to make a M&S medium (Murashige and Skoog). And I had to make 3 "bottles" of medium and then add those together. But why did I need to make those "in between solutions" (stocksolutions?)

the ingredients are:

NH4NO3
KNO3
CaCl2•2H2O
MgSO4•7H2O
KH2PO4
H3BO3
MnSO4•H2O
ZnSO4•H2O
KI
NaMoO4•2 H2O
CuSO4•5H2O
CoCl2•6H2O
FeSO4•7H2O
Na2EDTA•2H2O
myo-inositol
nicotinic acid
thiamine•HCl
pyridoxine•HCl
glycine
sucrose
agar

I think it has something to do with preventing precipitation? But what components would precipitate then?
(maybe iron and phosphates?)


(and I can not remember what the 3 different stock solutions were I had to mix seperatly before putting it all together)

[rjb]

Polleke,

Would be interesting to know what in each of the 3 bottles. In terms of precipitation, I've always thought that the big issue are PGR's more than anything... Auxins and cytokinins are weak acid/bases respectively and don't really like going into aqueous solution...

R

[Polleke]

Polleke,

Would be interesting to know what in each of the 3 bottles. In terms of precipitation, I've always thought that the big issue are PGR's more than anything... Auxins and cytokinins are weak acid/bases respectively and don't really like going into aqueous solution...

R

Hallo Rjb,

solution1:
MgSO4•7H2O
KNO3
NH4NO3

Solutionb2:CaCl2•2H2O

Solution3:
KH2PO4

solution 4:
H3BO3
MnSO4•H2O
ZnSO4•H2O
KI
NaMoO4•2 H2O
CuSO4•5H2O
CoCl2•6H2O

solution5:
FeNaADTA was used in stead of FeSO4•7H2O

and vitamines
myo-inositol
nicotinic acid
thiamine•HCl
pyridoxine•HCl
glycine




I only added the hormones after autoclaving..

And it was the solutions 1, 2 and 3 I had to prepare in diffrent bottles to prevent precipitation.
(after this I simply had to put them toghether with the rest , solutions 4, 5 and vitamines were just seperated  because those where allready in stock)

[Polleke]

Polleke,

Would be interesting to know what in each of the 3 bottles. In terms of precipitation, I've always thought that the big issue are PGR's more than anything... Auxins and cytokinins are weak acid/bases respectively and don't really like going into aqueous solution...

R

I posted the bottles... No idea?