[issh]

Hi,

I am doing an experiment, by adding 5 mL of 10% Urea Solution and 2mL 5% Urease solution and 2mL of 0.1M Acetic Acid

You obtain the following:


      Urease
Urea    Ammonia + Carbon Dioxide


(NH2)2CO + H2O → CO2 + 2NH3


I am wondering if the amount of CO2 is large that can be quantitatively analysed by counting the amount of CO2 bubbles produced through the reaction.


Thanks and greatly appreciated

[Honclbrif]

Maybe not by "counting the bubbles", but certainly by measuring the volume of evolved gas. For this you would use an eudiometer, which is essentially an upside down graduated cylinder.

[OC pro]

Or bubbling it through a solution of barium hydroxide giving the very insoluble barium carbonate which can be analyzed gravimetrically.