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disulfide bonds
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stasia222:
Hi, I need to know how or what, other than using Glutathione, can break disulfide bonds at a neutral pH.
Thanks
Stacey
lemonoman:
To break the disulfide bond of cystine and form a thiol at each sulfur, they suggest added exces of 2-mercaptoethanol.
See this VERY general mechanism:
from http://www.biochemj.org/bj/357/0343/bj3570343.htm
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