Hi guys!
Needing help with a Biochemistry question -
'Express Vmax in terms of concentration change per minute, using your answer from Question 1 to convert absorbance change to concentration change'
Question 1 was working out the molar extinction coefficient, which I calculated to be 3.12 l mole^-1 cm^-1.
I also think I've got Vmax right, which came to 0.4 - I got this from my Lineweaver-Burke plot by looking at where the line intercepted the y-axis (2.35) then did 1 / 2.35 = 0.4.. have I done this right?
Can anyone give me some steps on how to figure out the above question about concentration change per minute?
Thanks for any advice!