March 28, 2024, 07:42:16 PM
Forum Rules: Read This Before Posting


Topic: Biotin modifications and reactions  (Read 4878 times)

0 Members and 1 Guest are viewing this topic.

Offline calim3ntry

  • New Member
  • **
  • Posts: 5
  • Mole Snacks: +0/-2
Biotin modifications and reactions
« on: December 06, 2011, 05:53:08 PM »
Need some advice whether this is even possible. I'm trying to design a biotin that is substituted on one of the N of the ring with a good leaving group. I've seen a bis-N-alkylated (+)-biotin derivative, but I only want one group modified. Then, on the tail, I'd like to have a His, or some functional group containing a His. I then need a good maybe nucleophile to attack aforementioned leaving group so the biotin "head" could be free to then bind to a streptavidin labeled signal. Basically, I need to keep the "head" protected/anchored with the first modification until I introduce the attacking group to then do some kind of substitution reaction to displace it.

Offline Mitch

  • General Chemist
  • Administrator
  • Sr. Member
  • *
  • Posts: 5298
  • Mole Snacks: +376/-3
  • Gender: Male
  • "I bring you peace." -Mr. Burns
    • Chemistry Blog
Re: Biotin modifications and reactions
« Reply #1 on: December 06, 2011, 06:39:05 PM »
Don't spam us with the same topic in multiple forums!
Most Common Suggestions I Make on the Forums.
1. Start by writing a balanced chemical equation.
2. Don't confuse thermodynamic stability with chemical reactivity.
3. Forum Supports LaTex

Offline calim3ntry

  • New Member
  • **
  • Posts: 5
  • Mole Snacks: +0/-2
Re: Biotin modifications and reactions
« Reply #2 on: December 13, 2011, 11:54:32 AM »
Sorry, didn't know. Was looking for help all over.

Sponsored Links