Hi there,

I was just going through my old analytical chem book, and i was just flipping through and saw this problem

"Calculate how much of each of the stock reagents listed below should be used to make 100mL of homogenization buffer containing:

10mM Tris pH8.6(dilute 50mM stock)

1mM PMSF (dilute 100mM stock)

1mM EDTA (dilute 20mM stock)

0.2uM pepstatin A (dilute 20uM stock)

1mM 2-mercaptoethanol (dilute 7.1M stock)

okay so this is what i get from this, with these 5 ingredients, all of these need to add up to 100mL

is this problem as simple as using Molarity=Moles/Volume or using C1xV1=C2xV2

I would appriciate it if someone can direct me in the right direction, on how to approach this problem

Thx