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Topic: Testing Nitrates in feed samples using ISE  (Read 2014 times)

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Offline Nutri

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Testing Nitrates in feed samples using ISE
« on: March 19, 2012, 02:48:22 PM »
Hello, I am setting up a Nitrates testing methodology but need some help with appropriate sample prep.

I have a Nitrates Ion Selective Electrode probe and bench top meter (also displays sample's temperature and pH.)
We are testing feed samples (hay, silages, grains, total mixed rations, etc) for Nitrates (% Nitrates and ppm Nitrates-Nitrogen.)

We receive the feed samples fresh then we dry and grind them.

The probe came with ISA packets (Nitrate Ionic Strength Adjustor powder) where you will add one packet of ISA to 25mL of sample or standard.

We have a mechanical shaker
We have filter paper, lab glassware

What should the sample prep be?
How much dry, ground sample diluted in how much water?
I hope no digestion  / extraction is necessary?
Should we shake the samples? for about 20 to 30 min?
Then filter?
Add ISA before or after filtration?

Thank-you very much!
« Last Edit: March 19, 2012, 03:08:04 PM by Nutri »

Offline fledarmus

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Re: Testing Nitrates in feed samples using ISE
« Reply #1 on: March 19, 2012, 02:56:02 PM »
It looks like this is what you are looking for:

http://www.nico2000.net/analytical/nitrate.htm

Except for the ISA anyway. Not sure what those are supposed to do.

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