April 29, 2024, 01:54:49 AM
Forum Rules: Read This Before Posting


« previous next »
Pages: [1]   Go Down

Topic: Absorption maxima at 230, 260 and 410 nm?  (Read 2784 times)

0 Members and 1 Guest are viewing this topic.

Offline Aquatica

  • Very New Member
  • *
  • Posts: 1
  • Mole Snacks: +0/-0
Absorption maxima at 230, 260 and 410 nm?
« on: March 11, 2012, 07:34:14 AM »
Hi all,
I have a mathanol extract (from bacterial culture) showing absorption maximum at 231 nm and two smaller ones at 260 and 415 nm. Any ideas what king of compounds that might be? Thanks in advance!
Logged

Offline Arkcon

  • Retired Staff
  • Sr. Member
  • *
  • Posts: 7367
  • Mole Snacks: +533/-147
Re: Absorption maxima at 230, 260 and 410 nm?
« Reply #1 on: March 11, 2012, 07:46:48 AM »
This is a very tough problem to work with.  UV-Vis spectroscopy isn't very useful for predicting a compound's structure.  Since you said you've extracted with methanol, presumably from bacterial culture solids, the 260 nm absolution indicates something like a pyrimidine, but may really be anything.
Logged
Hey, I'm not judging.  I just like to shoot straight.  I'm a man of science.

Offline Pradeep

  • Regular Member
  • ***
  • Posts: 95
  • Mole Snacks: +5/-3
Re: Absorption maxima at 230, 260 and 410 nm?
« Reply #2 on: March 11, 2012, 10:48:12 AM »
You should separate the compounds and take a NMR and Lc or GC/MS and IR also. Then you  will be able to elucidate the structure with more confidence.
Logged
Pages: [1]   Go Up
« previous next »
Sponsored Links