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Topic: Amino acid hydrolization  (Read 4627 times)

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Offline Kork

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Amino acid hydrolization
« on: March 23, 2012, 05:38:58 AM »
When a peptide is hydrolysed Aspartate is found, but as far as I understand that doesnt mean that Aspartate was in the peptide, but another amino acid could have transformed into Aspartate. Is it possible to know which amino acid? To me the most similar one is Asparagine.

Offline fledarmus

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Re: Amino acid hydrolization
« Reply #1 on: March 23, 2012, 07:24:55 AM »
1) What type of chemical bond is broken when a peptide is hydrolyzed? What is the general reaction and what are the products of that reaction?

2) Where does that type of bond exist in the sidechains of naturally occurring amino acids? What product(s) would you get from performing the reaction on those sidechains as well as the backbone of the peptide?

Offline AWK

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Re: Amino acid hydrolization
« Reply #2 on: March 23, 2012, 07:40:17 AM »
Quote
Is it possible to know which amino acid? To me the most similar one is Asparagine.
Yes, Asn also forms Asp during hydrolysis.
AWK

Online Babcock_Hall

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Re: Amino acid hydrolization
« Reply #3 on: March 23, 2012, 09:08:16 AM »
Kork, If you find glutamate after hydrolyzing a peptide, what does that suggest to you?

Offline Kork

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Re: Amino acid hydrolization
« Reply #4 on: March 23, 2012, 09:21:14 AM »
I dont still dont get it.

Online Babcock_Hall

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Re: Amino acid hydrolization
« Reply #5 on: March 23, 2012, 09:58:04 AM »
Maybe we should go back to aspartate for a moment.  Did you understand that, or are you still unsure about what people said?

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