I am running an experiment measuring the rate of the break down of Aspartame to DKP in various buffers (Citrate&Phosphate) at different temperatures (40,50,60 C).
I have run HPLC and have various Area values for different times of the reaction and plotted a graph of Ln(area) vs Time to get a nice linear graph.
In my experiments 'instructions' it states that The area at time t, At, must be corrected for the small reading after the aspartame has completely reacted, A∞ (i.e., [At-A∞]).
It then goes on to say I should plot a graph of Ln[At-A∞] vs time to obtain Kobs.
My Confusion is this... Surely the area when the aspartame is completely reacted (obtaining this data by extrapolating my graph) will be 0 and so At-A∞ will be no different from At on its own?